1型、2型和成人隐匿型自身免疫糖尿病的细胞内IFN-γ和IL-13免疫反应:Action LADA 6
发布时间：2012-10-08 15:30:10浏览次数：3380次来源：中南大学湘雅二医院 摘自:Cytokine. 2012, 58(2):148-51.
1型糖尿病和成人隐匿型自身免疫糖尿病（LADA）是由于胰岛β细胞免疫性破坏引起的。LADA与1型糖尿病和2型糖尿病患者遗传特征部分类似，主要与1型糖尿病遗传关联。LADA与1型糖尿病患者具有相似的免疫学特征，都有自身抗体和系统性细胞因子如IL-1RA,IL-6和TNF-α。LADA患者临床上类似于2型糖尿病，比1型糖尿病临床表现轻微，其机理仍然不明。本研究旨在比较LADA和1型糖尿病患者促炎性（干扰素-γ，IFN-γ）和抗炎性（白介素-13，IL-13）T细胞水平。
本研究运用酶联免疫斑点技术（ELISPOT）,在35例1型糖尿病、59例2型糖尿病、23例LADA患者以及42例正常对照中，检测了的16种（自身）抗原特异性刺激后外周血反应性T细胞分泌的IFN-γ和IL-13水平。我们发现，LADA和1型糖尿病患者对自身抗原刺激、阳性对照或有丝分裂原刺激后产生的反应性IFN-γ和IL-13T细胞频率没有统计学差异。糖尿病各组患者的自身抗原特异性的T细胞免疫反应水平较低。1型糖尿病、LADA或2型糖尿病患者对破伤风类毒素刺激的免疫反应水平相似，都比健康对照的反应性T细胞的IL-13水平低（P＜0.05）。1型糖尿病、LADA以及2型糖尿病，都与较弱的回忆性抗原破伤风类毒素刺激后T细胞反应水平相关。进一步分析表明，破伤风类毒素刺激后反应性T细胞的IFN-γ和IL-13水平与糖尿病病程、年龄及BMI无关。
所以本研究认为，通过检测有丝分裂原和回忆性性抗原刺激后的T细胞内IFN-γ和IL-13 水平，不能区分LADA和1型糖尿病患者。这些结果扩展了先前的发现，即LADA和1型糖尿病的系统性细胞因子和/或趋化因子以及体液免疫水平是相似的。
Cellular interferon-c and interleukin-13 immune reactivity in type 1, type 2 and latent autoimmune diabetes: Action LADA 6
Objective: Type 1 diabetes and latent autoimmune diabetes in adults (LADA) are thought to result from immune-mediated b-cell destruction. It remains unclear why LADA is clinically less severe compared to type 1 diabetes. This study aimed to compare the pro-inflammatory (interferon-c, IFN-c) and anti-inflammatory (interleukin-13, IL-13) T-cell responses in humans with LADA and type 1 diabetes.
Research design and methods: IFN-c and IL-13 T-cell responses to a panel of 16 (auto)-antigens were tested using an enzyme linked immune-spot technique and peripheral T-cells from 35 patients with type 1 diabetes, 59 patients with type 2 diabetes, 23 LADA patients, and 42 control subjects.
Results: LADA and type 1 diabetes patients did not display any statistically significant differences in the frequency of IFN-c or IL-13 responses to auto-antigenic stimuli, positive control or mitogen. Overall very low T cell reactivity to autoantigens was detected in all groups. IL-13 responses but not IFN-c responses to recall antigen tetanus toxoid were higher in healthy control subjects compared to patients with type 1 or type 2 diabetes or LADA (P < 0.05). Diabetes, independent of type, was associated with weaker response to recall antigen tetanus toxoid.
Conclusions: LADA patients are indistinguishable from type 1 diabetes patients for cellular IFN-c and IL-13 responses upon mitogen and recall antigen stimulation. These results extend previous findings showing that systemic cytokine/chemokine and humoral responses in type 1 diabetes and LADA are similar.
链接: http://www.sciencedirect.com/science/article/pii/S1043466612000051
